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Sep 30, 2024
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University of Alberta Calendar 2023-2024 [ARCHIVED CATALOG]
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MMI 391 - Current Methods in Molecular Biology
Course Career Undergraduate
Units 3
Approved Hours 0-0-8
Fee index 6
Faculty Medicine and Dentistry
Department Med Microbiology & Immun
Typically Offered first term
Description
This laboratory course will introduce the student to common techniques in molecular biology. Through a series of experiments, students will clone and express the genes for bioluminescence from the light-emitting bacterium Vibrio fischeri. Technical skills will include: preparation and quantification of genomic and plasmid DNAs, screening a genomic library, restriction mapping, amplification of DNA fragments using the polymerase chain reaction, Southern blotting, expression and purification of proteins, and detection of proteins by Western blotting. Prerequisites: IMIN 200 and departmental consent. Priority given to Honors students in the IMIN program. Credit may be obtained for only one of BIOL 391, IMIN 391 or MMI 391.
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